Researchers first identified 5mC-modifying enzyme (CMD1) CMD1 as a TET homologue in Chlamydomonas reinhardtii. The enzyme catalyses the conjugation of a glyceryl moiety to the methyl group of 5mC, with vitamin C as the donator of the glyceryl moiety.
Furthermore, by using CRISPR-Cas9 technology, they concluded that the glyceryl 5mC modification could regulate expression of photoprotection gene LHCSR3 through demethylation, thus influence acclimation to high light.
The discovery of the new mechanism in Chlamydomonas reinhardtii is of great importance to the study of eukaryotic DNA methylation. The modification may be used for DNA technologies such as the genome-wide mapping of 5mC because of its selectivity. As to Chlamydomonas reinhardtii, the successful application of CRSIPR-Cas9 in this study will be helpful to other genetic studies.
Researchers at the Shanghai Center for Plant Stress Biology, Center for Excellence in Molecular Plant Sciences of the Chinese Academy of Sciences, have uncovered a novel mechanism that maintains DNA methylation and genome stability in plants.
A team led by Profs Andrew TESCHENDORFF and WANG Sijia from Shanghai Institute of Nutrition and Health demonstrated that it is possible to use a computational approach to infer cell-type specific DNAm changes, thus avoiding the need for extremely laborious and expensive c...
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