/   Home   /   Newsroom   /   Research News

Oleaginous Yeast Serves as β-carotene Cell Factory

Apr 20, 2017     Email"> PrintText Size

β-carotene is used as a color additive or nutritional supplement for humans and animals, and is commercially produced by chemical synthesis or through Blakeslea trispora fermentation. Previous studies have focused on engineered Escherichia coli and Saccharomyces cerevisiae for heterologous β-carotene production.

Prof. YANG Sheng and colleagues at Shanghai Institute of Plant Physiology and Ecology of Chinese Academy of Sciences developed a series of genome editing tools for Y. lipolytica. And they generated an efficient biosynthetic pathway using strong promoters and multiple gene copies for each of the 12 steps.

In their study, using fed-batch fermentation with an optimized medium, the engineered pathway could produce 4 g/L β-carotene, which is stored in lipid droplets within engineered Y. lipolytica cells.

This work demonstrated that Y. lipolytica could be an industrially relevant platform for hydrophobic terpenoid production.

The study was published online in Metabolic Engineering and was supported by Chinese Academy of Sciences and the Ministry of Science and Technology of China. 


(Editor: LIU Jia)

Related Articles

anthocyanin;gibberellic acid;DELLA;biosynthesis

Researchers Reveal New Regulatory Mechanisms of Anthocyanin Biosynthesis

May 13, 2016

Through analyzing the molecular mechanism underlying gibberellic acid (GA)-regulated anthocyanin biosynthesis, Prof. HANG Jirong's lab at the Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences revealed tha...

Contact Us

Copyright © 2002 - Chinese Academy of Sciences