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LIN-15B Unveiled as Key Transcriptional Repressor Regulating Autophagy in C. elegans Development

Apr 10, 2025

On April 2, researchers led by Prof. ZHANG Hong from the Institute of Biophysics of the Chinese Academy of Sciences, published a new study in Autophagy, uncovering a novel mechanism by which autophagy activity is regulated in Caenorhabditis elegans. They identified IMPORTIN-13 (IPO-13) as a crucial factor that controls autophagy and lysosomal function by mediating the nuclear transport of the transcription factor LIN-15B.

The key steps of the autophagy process include autophagosome formation, fusion of the autophagosome with the lysosome, and lysosomal degradation. While transcriptional regulation of autophagy-related genes is known to play a central role in modulating this process, it has remained unclear whether additional transcriptional regulators are systematically involved during C. elegans development.

LIN-15B contains a THAP domain and plays roles in chromosome segregation, chromatin modification, and transcriptional regulation. The researchers found that IPO-13 acts as the nuclear import receptor for LIN-15B, transporting LIN-15B from the cytoplasm into the nucleus.

Under physiological conditions, LIN-15B is predominantly localized in the nucleus, where it suppresses the expression of genes related to the autophagy-lysosome pathway, maintaining low transcriptional levels of autophagy genes to ensure basal autophagy activity.

In ipo-13 mutants or starved worms, LIN-15B fails to efficiently enter the nucleus, leading to reduced nuclear levels of LIN-15B and consequently upregulated expression of autophagy genes, resulting in increased autophagic flux.

The study identified LIN-15B as a critical transcription factor regulating autophagy activity during C. elegans development, with its regulatory function depending on the nuclear transport protein IPO-13 to shuttle it from the cytoplasm into the nucleus-a process that is modulated by the organism's nutritional status.

This research uncovers a novel mechanism by which cells sense nutritional cues to regulate autophagy activity.

Model showing that IPO-13-mediated cytoplasm-to-nucleus transport of LIN-15B controls autophagy activity. (Image by ZHANG Hong's group)

Contact

ZHANG Hong

Institute of Biophysics

E-mail:

T16G12.6/IMPORTIN 13-mediated cytoplasm-to-nucleus transport of the THAP transcription factor LIN-15B controls autophagy and lysosome function in C. elegans

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