There exists a variety of lysosomes in a single cell, such as autophagy lysosome and endolysosome. Studying the specificity of different types of lysosomes in the process of aging can provide new approaches for the development of drug targets for anti-aging and the treatment of aging related diseases. Due to technical limitations, it is not possible to detect single lysosome metabonomics, let alone to study cell aging from the perspective of single lysosome metabonomics.
The researchers in this study established the single lysosomal mass spectrometry platform based on single lysosome patch and ultra-low speed induction electrospray ionization mass spectrometry. The platform realized the detection of metabolites directly without any pretreatment in cells, and more accurately retained the information of metabolites in lysosomes.
They then conducted in-depth research on the metabolic heterogeneity of various types of lysosomes in the process of cell aging on the platform.
Based on the single lysosome metabonomics, lysosomes from at least four cell sources were divided into five subgroups, including endocytic lysosomes and autophagic lysosomes and metabolic changes of various lysosomes in the aging process are studied.
This study indicates that there exists lysosome type specificity in the changes of lysosome metabolism in the process of cell aging.
Jane FAN Qiong
University of Science and Technology of China
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