Splicing Factor SRSF10 is an Essential Regulator during Adipogenesis
May 04, 2014 Email"> PrintText Size
Alternative splicing is a major mechanism that controls gene expression and increases protein diversity in mammalian cells. Increasing evidence indicates that precise regulation of alternative splicing is responsible for tissue type and developmental stage determination. However, little is known about roles played by splicing factors in these processes.
A research group led by FENG Ying, professor of the Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, found that SRSF10, a typical member of SR splicing factor family, plays an essential role in adipogenesis. They observed that development of axillary subcutaneous white adipose tissue is greatly impaired in SRSF10 KO mice. At the same time, SRSF10 knockdown can significantly reduce hormone-induced adipogenesis in primary MEF and C3H10 cells. RNA-seq analysis, performed using primary MEF cells, showed Lipin1 is one target gene of several SRSF10-affected splicing events implicated in adipogenesis. SRSF10 binds to cis element in exon8 of Lipin1 to repress the exon7 inclusion, generating Lipin1α isoform in wild type cells. Deletion of SRSF10 promotes exon7 inclusion to generate Lipin1β isoform. Consistent with this observation, overexpression of Lipin1α in SRSF10 deficient C3H10 cells can recover adipogenesis more than Lipin1β. These data indicate that Lipin1α is more important in initial adipocyte differentiation
The article, entitled “SRSF10 regulates alternative splicing and is required for adipocyte differentiation” and published ahead of print in the April Molecular and cellular Biology, demonstrated that splicing factor SRSF10 plays a crucial role in adipose differentiation and revealed new alternative splicing targets of SRSF10 in vivo.
This study was funded by grants from
CONTACT:
FENG Ying, M.D. & Ph.D., Principal Investigator
Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences,
Shanghai 200031, China.
Tel: 86-21-54920965;
Fax: 86-21-54920291;
E-mail: fengying@sibs.ac.cn
Alternative splicing is a major mechanism that controls gene expression and increases protein diversity in mammalian cells. Increasing evidence indicates that precise regulation of alternative splicing is responsible for tissue type and developmental stage determination. However, little is known about roles played by splicing factors in these processes.
A research group led by FENG Ying, professor of the Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, found that SRSF10, a typical member of SR splicing factor family, plays an essential role in adipogenesis. They observed that development of axillary subcutaneous white adipose tissue is greatly impaired in SRSF10 KO mice. At the same time, SRSF10 knockdown can significantly reduce hormone-induced adipogenesis in primary MEF and C3H10 cells. RNA-seq analysis, performed using primary MEF cells, showed Lipin1 is one target gene of several SRSF10-affected splicing events implicated in adipogenesis. SRSF10 binds to cis element in exon8 of Lipin1 to repress the exon7 inclusion, generating Lipin1α isoform in wild type cells. Deletion of SRSF10 promotes exon7 inclusion to generate Lipin1β isoform. Consistent with this observation, overexpression of Lipin1α in SRSF10 deficient C3H10 cells can recover adipogenesis more than Lipin1β. These data indicate that Lipin1α is more important in initial adipocyte differentiation
The article, entitled “SRSF10 regulates alternative splicing and is required for adipocyte differentiation” and published ahead of print in the April Molecular and cellular Biology, demonstrated that splicing factor SRSF10 plays a crucial role in adipose differentiation and revealed new alternative splicing targets of SRSF10 in vivo.
This study was funded by grants from
CONTACT:
FENG Ying, M.D. & Ph.D., Principal Investigator
Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences,
Shanghai 200031, China.
Tel: 86-21-54920965;
Fax: 86-21-54920291;
E-mail: fengying@sibs.ac.cn
CAS Institutes
There are 124 Institutions directly under the CAS by the end of 2012, with 104 research institutes, five universities & supporting organizations, 12 management organizations that consist of the headquarters and branches, and three other units. Moreover, there are 25 legal entities affiliated and 22 CAS invested holding enterprisesThere are 124 I...>> more
Contact Us
Chinese Academy of Sciences
Add: 52 Sanlihe Rd., Xicheng District, Beijing, China
Postcode: 100864
Tel: 86-10-68597592 (day) 86-10-68597289 (night)
Fax: 86-10-68511095 (day) 86-10-68512458 (night)
E-mail: cas_en@cas.cn