/   Home   /   Newsroom   /   Research News

Scientists Decode the First in in vivo RNA Structurome in Rice

Feb 09, 2018     Email"> PrintText Size

RNA is an essential molecule in the flow of genetic information. It forms complicated secondary and tertiary structure to perform biological functions. Non-coding RNAs (eg. rRNAs, tRNAs and snRNAs) adopt very stable and specific secondary structures in splicing and translation processes.
The secondary structure of mRNAs has also been found to play an important role in post-transcriptional and translational regulation. However, it is difficult to decode RNA secondary structure in vivo.
While functional genomics in rice have achieved many impressive improvements in recent years aiming for better evolutionary studies and rice production, the nature of RNA secondary structure in rice remains poorly understood.
Recently, Dr. CAO Xiaofeng's group at Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, collaborated with Yiliang Ding's group in John Innes Centre in the United Kingdom, decoded the first in vivo RNA structurome in rice.
The researchers successfully established the Structure-seq method by dimethyl sulfate (DMS) in rice and generated libraries with high quality and depths for the first time.
They found that several similar RNA structural features in rice appeared to impact multiple aspects of RNA function and processing, which is similar to those in Arabidopsis.
Moreover, they found that RNAs with higher N6-methyladenosine (m6A) modification tends to have less RNA structure, especially in 3'UTR.
Lastly, sequence identity and RNA secondary structure similarity comparison between rice and Arabidopsis revealed a weak correlation between them.
Gene Ontology enrichment analyses implied that RNA structure evolves differently for various biological processes to maintain robustness in development and flexibility in adaptation during angiosperm evolution.
Their study provided a separate layer of RNA structure-function analyses for understanding the gene function in addition to the traditional way of sequence-function analyses.
This study has been published online in Molecular Plant entitled "Rice in vivo RNA structurome reveals RNA secondary structure conservation and divergence in plants".
This research was supported by Biotechnology and Biological Sciences Research Council (BBSRC) and University of the Chinese Academy of Sciences (UCAS) Joint PhD Training Program. 
Figure. In vivo RNA Structurome Evolution (Image by IGDB)


(Editor: ZHANG Nannan)

Related Articles

rice;chilling tolerance;OsbHLH002/OsICE1;OsMAPK3;phosphorylation;ubiquitination

Chinese Scientists Reveal a Novel Signaling Pathway for Chilling Tolerance in Rice

Dec 19, 2017

The research team guided by Prof. CHONG Kang from Institute of Botany of the Chinese Academy of Sciences reveals new mechanism of chilling tolerance mediated by OsMAPK3-OsbHLH002-OsTPP1 in rice.

rice;WTG1;grain size and shape;grain weight;cell expansion;

The Otubain-like Protease WTG1 Regulates Grain Size and Shape in Rice

Jul 03, 2017

A recent study by Dr. LI Yunhai's group at the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, in collaboration with Dr. Wang Dekai at Zhejiang Academy of Agricultural Sciences, isolated a wide and thick grain 1 (wtg1-1...

rice;rice domestication;radiocarbon dating;Shangshan;phytolith-occluded carbon;LU Houyuan

Radiocarbon Dating of Phytolith Traces Rice Domestication to 10,000 Years Ago

Jun 01, 2017

Rice (Oryza sativa L.) is one of the world's most important staple foods, as it sustains more than half the global population. Rice farming has provided an important material and cultural basis for the development and prosperity of Chinese civilization...

Contact Us

  • 86-10-68597521 (day)
    86-10-68597289 (night)
  • 86-10-68511095 (day)
    86-10-68512458 (night)
  • cas_en@cas.cn
  • 52 Sanlihe Rd., Beijing,
    China (100864)

Copyright © 2002 - Chinese Academy of Sciences