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New Study: Low Concentration of CO Could Protect Bystander Cell in Cell Cluster Mode

Feb 16, 2017     Email"> PrintText Size

Radiation-induced bystander effect (RIBE) is defined that the irradiated cells release number of factors to cause cell proliferation, genetic damage, gene mutation and even tumor formation in the near or even abs-copal non-irradiated tissue.

And carbon monoxide (CO) in low concentration is an important physiological signal molecule and had been proved potentially applicable in clinic. The possible signal pathways which are involved in the RIBE have been found to be modulated by CO.

Lately, a new study reported by Pro. HAN Wei’s study team at center of medical physics and technology, Hefei Institutes of Physical Science of the Chinese Academy of Sciences revealed that low concentration of exogenous carbon monoxide could modulate RIBE in a concentration dependent manner in mammalian cell cluster mode.

A low concentration of CORM-2 (30 μM), a kind of CO-releasing molecule, was sufficient to protect the bystander cells, which decreased DNA double-strand breaks, micronuclei and cell proliferation into the background level.

The mechanism shows that low concentration of CO modulated RIBE via decreasing the expression level of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), which are critical signal molecules in the RIBE. The results can provide the data base and hints for the potential application of CO in clinic.

Actually, in the previous study, HAN’s group has revealed that CO in low concentration protected the bystander cell from the RIBE signals in two-dimensional monolayer cell model (Mutation research, 2014).

However, RIBE in two-dimensional monolayer cell model as a single simplified cultivation system is different from RIBE in three-dimensional model, the results of three-dimensional model is considered to be close to the physiological condition. Their finding, this time, may shed new light on RIBE.

This study was detailed in International Journal of Molecular Sciences titled Low Concentration of Exogenous Carbon Monoxide Modulates Radiation-Induced Bystander Effect in Mammalian Cell Cluster Model.

This research was supported by the National Natural Science Foundation of China, the Natural Science Fund of Anhui Province and the Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology


Figure 1. Schematic representation of multicellular cluster. (Image by WU Wenqing and NIE Lili) 


Figure 2. CO decreases double-strand breaks (DSBs) in bystander cells. (Image by WU Wenqing and NIE Lili) 


Figure 3. CO decreases the expression of iNOS or COX-2 in the bystander cells. (Image by WU Wenqing and NIE Lili) 


(Editor: ZHANG Nannan)



Center for Medical Physics and Technology

Phone: 86-551-65595100
E-mail: hanw@hfcas.ac.cn

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